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Bioinformatics : Recent Questions and Answers (Page 13)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

How to fit all genes (labels) in chromosome ideogram plot made by RCircos package?

I am using Rcircos to make a chromosome ideogram plot for my gene list (n = 45). However, I am getting this error: Not all labels will be plotted....

Asked on 08/21/2021

2 answer

How to add cluster name to metadata in Seurat?

I'm working on a Seurat object and want to name the clusters according to 2 values alone (yes/no). So I want to add a new column to metadata and annotate...

Asked on 08/21/2021 by Najeha Mohamed

1 answer

How to calculate the number of peaks that are upstream/downstream of some other peaks

I have 3 histone marks,I have used Macs2 for peak calling and diffBind to analyze the peaks. I was wondering if you know any way to calculate the peak numbers...

Asked on 08/21/2021 by Mariam

1 answer

How to subset genes and its nested features from a GFF file using a gene list

I would like to subset a GFF file (gene and nested features) from a gene list. The GFF file looks like this##gff-version 3Scaffold_1 JGI...

Asked on 08/21/2021 by Felipe Almeida

2 answer

Standard for when to collapse phylogenetically uninformative nodes

I have been creating phylogenetic trees using RAxML (using Boostrap + ML) and I end up with a bestTree file, outputted in newick format. When I open up the tree...

Asked on 08/21/2021 by Ace8910

1 answer

P-value correction when evaluating correlation between gene and miRNA expression

First of all I apologize without the question is very basic, I am taking my first steps in bioinformatics.Data informationWe are evaluating the correlation (using the Pearson, Kendall or Spearman...

Asked on 08/19/2021

1 answer

How to run MaxQuant in command line mode?

MaxQuant is a software package for mass spectroscopy and proteomics. There is a windows version and a linux version. To run on linux you have to use a program that...

Asked on 08/19/2021

2 answer

Why does the FASTA sequence for coronavirus look like DNA, not RNA?

I'm looking at a genome sequence for 2019-nCoV on NCBI. The FASTA sequence looks like this:>MN988713.1 Wuhan seafood market pneumonia virus isolate 2019-nCoV/USA-IL1/2020, complete genomeATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTG... ...TTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGACAAAAAAAAAAAACoronavirus...

Asked on 08/19/2021 by jameshfisher

4 answer

How identifiable are human omics data and how to mitigate their identifying features?

Say a database were to store human omics datasets. The human subjects are known and the sample size is rather small in size initially (n=500). The database contains genomics, transcriptomics,...

Asked on 08/19/2021 by clove444

2 answer

How to extract metadata from NCBI's experiment?

I want to extract metadata from experiment SRX1596422. I have sratoolkit and Entrex direct but not sure what line of code to use. Here's the link for the experiment: ...

Asked on 08/19/2021 by Ophelia Vallee

1 answer

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