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Bioinformatics : Recent Questions and Answers (Page 11)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

maftools tcgaCompare plot: x-axis labels getting clipped

I have a {maftools} plot: whatever I try to save that completely I see the down part of plot is trimmed like ...

Asked on 08/22/2021

1 answer

scRNA-Seq: Account for sequencing depth and gene length?

Task: Normalize a single-cell RNA-Seq dataset to account for sequencing depth and gene-length. For UMI-count based protocols (like 10x) that don't suffer from gene-length biases, there are various methods developed...

Asked on 08/22/2021 by Bluescreen

0 answer

How to get a list of genes corresponding to the list of SNPs (rs ids)?

Is there a way for me to get a list of genes given a list of SNP rs ids? I found several questions asked with a similar goal years ago,...

Asked on 08/22/2021

6 answer

Merge / Reconciliate several de novo transcriptome assemblies with different kmers

I am building a De Novo transcriptome reference assembly for an eukaryotic organism for which I have a genome. I've created several assemblies with rnaSpades using different kmer sizes (19...

Asked on 08/22/2021 by Pitagoras Alves

2 answer

How to extract Genbank sequence and identify ORFs from the sequence in R?

I usedape::read.GenBankand then used the functionORFik::findORFsto find ORFs in the sequence of interest. But the former one results in a DNAbin class format and the latter one requires...

Asked on 08/22/2021 by Prradep

0 answer

How are the values of prop.part() and prop.clades() calculated?

Consider the following dataset:fictional.df <- data.frame(L1 = c(0,0,0,0,0,0,0,0), ...

Asked on 08/22/2021 by Namenlos

0 answer

Principle of TMT Tags in Multiplex Proteomics

I am new to proteomics research and analyzing mass-spec data. I am trying to learn more about the theory/principle of TMT tagging (a type of isobaric labeling) coupled with LC-MS/MS...

Asked on 08/22/2021 by Osaama Shehzad

1 answer

How does picard's MarkDuplicate handle unmapped reads?

Our BAM files are created according to a "lossless" alignment procedure [1] from the Broad Institute GATK documenation and involves re-adding the unaligned/unmapped reads into an aligned...

Asked on 08/22/2021 by init_js

1 answer

Can Maven (mass spectrometry software) read mzML files?

I did not find a documentation. Can Maven (mass spectrometry software) read mzML files?...

Asked on 08/22/2021 by Sören

1 answer

Java error when trying to run UBCG jar (java.lang.ClassNotFoundException)

I wanna install UBCG.JAR in ubuntu 18.04 but I cannot run the java File UBCG.JAR.when I type the code java -jar UBCG.JAR an error message appears.Exception in thread...

Asked on 08/22/2021 by Maloki

1 answer

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