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Bioinformatics : Recent Questions and Answers (Page 9)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

differential analysis of chip-seq data

I have several sets of chip-seq data. I called the peaks using Macs2. I am pretty new to the field and I will appreciate any help. I wanted to annotate...

Asked on 08/26/2021

3 answer

Coronavirus RNA structures?

Is there anything known about the RNA structures of coronaviruses? More specifically - do they have any interesting known structures in the translatable region, like RRE of HIV or the...

Asked on 08/26/2021

1 answer

Is it possible for coronavirus or SARS to be synthetic?

I have heard several conspiracy theories regarding the origin of the new coronavirus, 2019-nCov. For example that the virus and/or SARS were produced in a laboratory or were some variant...

Asked on 08/25/2021 by Sscheme

1 answer

Subsetting from seurat object based on orig.ident?

I am pretty new to Seurat. I want to subset from my original seurat object (BC3) meta.data based on orig.ident. however, when i use subset(), it returns with Error.ER_HER_P...

Asked on 08/25/2021

2 answer

Extract sequences from partial Header

I have a library I would like to split them based on their headers. Some are related to RNA and DNA. The header contains much information, but the most important...

Asked on 08/25/2021 by BioInfo

2 answer

How to reduce the occupied RAM when you are dealing with a very sparse matrix in a single-cell Experiment in R?

I'm dealing with a very large and sparse dataset and the first issues I met occurred when I tried to use quickCluster that reported me this error: ...

Asked on 08/22/2021

3 answer

Bacterial DNA at the tail of transcriptome reads. What does that mean?

I am assembling a transcriptome obtained from the Internet. The transcriptome was extracted from a human cancer tissue that had been previously grafted into a mouse. I have detected that...

Asked on 08/22/2021 by juanjo75es

2 answer

Chromatin accessibility level on promoters with different CpG ratio calculation

I want to categorize promoters based upon high, intermediate and low-CpG content (high-CpG-density promoters (HCPs), intermediate-CpG-density promoters(ICPs), and low-CpG-density promoters (LCPs)). So the data I have is for promoter which...

Asked on 08/22/2021 by krushnach Chandra

1 answer

What is the best way to get a large number of RNA seq data from SRA in Python without being denied access

I have the following code to download data from the SRA using multithreading in Python. After running this a few times now (for testing purposes), I keep getting denied access...

Asked on 08/22/2021

1 answer

Trying to download a large number of files from ENA programmatically

I am downloading a large number of files from the ENA in Python using Multithreading. As an example, to be more concrete, I tried downloading 12 files simultaneously using 12...

Asked on 08/22/2021

0 answer

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