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Bioinformatics : Recent Questions and Answers (Page 43)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

Downloading SRA Files from AWS

I want to download the original BAM files that the authors had uploaded to SRA. Normally, I would just use sam-dump, but the files are having issues that seem related...

Asked on 12/06/2020

1 answer

NCT search in PubMed via Entrez (python)

first time I post here so, please, be patient.So I am currently working with Entrez (Biopython) in order to retrieve the number of articles for a given disease/indication. My...

Asked on 12/05/2020 by Nutarelli Federico

0 answer

Comparing groups of 16S rRNA sequences - how to?

I have several groups of 16S rRNA sequences associated with taxonomic groups (let's call them A, B and C). The sequences within each group have a common ancestor and are...

Asked on 12/05/2020

1 answer

Relationship between the instantaneous rate of change of a continuous time Markov model and the rate of a global clock model?

If continuous-time Markov models contain a parameter q that denotes the instantaneous rate of change and a global/strict clock model is the overall rate of evolution, how are these two...

Asked on 12/04/2020

1 answer

load the phenotype data for ballgown

I am trying to reproduce the work of this paper[1], and I have run StringTie successfully, but after that I have to run...

Asked on 12/03/2020 by fatima

1 answer

using snakemake shadow rules to store temp files on local nodes

Snakemake is not really designed to store temporary files to local disk, but in the documentation, they suggest a workaround using "shadow rules". The problem I have...

Asked on 12/02/2020 by Kamil S Jaron

1 answer

Need help with Pairwise Alignment module in iterating over the alignment

from Bio import SeqIOfrom Bio import Alignref_seq_1 = SeqIO.read('C:/Users/King_Arthur/Desktop/ref_seq/segment 1/ref_seq_8.fasta','fasta')seq1 = SeqIO.read('C:/Users/King_Arthur/Desktop/file/segment 1/Myfile_1 (1).fasta','fasta')aligner = Align.PairwiseAligner()aligner.mode = 'global'aligner.match_score = 1aligner.mismatch_score = -2aligner.gap_score = -2alignments = aligner.score(ref_seq_1.seq , seq1.seq)print(alignments)for alignment...

Asked on 12/01/2020 by Neeleshwar Pandey

1 answer

Software for taxonomic assignment?

I have a couple of hundred bacterial sequences of 2-30 genes of interest each, recovered from metagenomics. None of them encode rRNA. Normally I'd just BLAST the one gene I...

Asked on 12/01/2020

2 answer

Any user friendly way to find rare mutations in whole genome raw?

Is there any user friendly way to find rare mutations in the individual human whole genome sequencing raw data? (from Dante, 30x coverage). To be more specific, I want to...

Asked on 11/29/2020 by musinn

1 answer

marge the matrix from computematrix of deeptools

I have several matrix from computematrix of deeptools. I need to merge two of them using "computeMatrixOperations cbind -m input1.mat.gz input2.mat.gz -o output.mat.gz"but I am running to error "/computeMatrixOperations.py",...

Asked on 11/28/2020

1 answer

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