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Bioinformatics : Recent Questions and Answers (Page 38)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

how can i search in p-blast against UNIPROT(not only swissprot)

i need to search for one protein against UNIPROT in BLAST for a selected organism.How can I do it? (SWSISSPROT+TREMBL)THANKS!!...

Asked on 01/22/2021

2 answer

How to get % similarity between strains and mutation files

I’m very new to python, and having some difficulty getting hang of some more complicated things I have multiple files which look like so:hCoV-19/Singapore/4/2020|EPI_ISL_410535|2020-02-03hCoV-19/USA/WA13-UW9/2020|EPI_ISL_413601|2020-03-02hCoV-19/USA/WA-UW142/2020|EPI_ISL_416680|2020-03-11Please be aware that the lines above...

Asked on 01/22/2021

1 answer

How to find Genotype, from list of mutations:lineage list?

I'm very new to python and I'm getting along nicely (I'd like to believe); however, I must be missing something here. I'm looking to read each and every file and...

Asked on 01/21/2021 by Theo Jones

1 answer

Why is it necessary to add hydrogen and delete water before protein-ligand docking?

What is the reason for adding hydrogen and removing unnecessary water molecules from the protein structure before protein-ligand docking? FYI, the tools I used for docking is GOLD....

Asked on 01/21/2021 by Zheng Keong Ng

3 answer

Find CRISPR PAM-sites with python

I am a college student and just started bioinformatics. I am trying to write a script (not for school) that finds all potential NGG and TTTN protospacer adjacent motif sequences...

Asked on 01/21/2021 by heibai

1 answer

Remove variants that do not map to human genome

[This question was also asked on Biostars] I received an hg38 VCF file that's had variants imputed with 1000 genomes. I've encountered some issues with the VCF;...

Asked on 01/20/2021 by John Rouhana

1 answer

Microarray Meta-Analysis and Cross-Platform

I have a microarray dataset with Agilent platform, all of the samples are cancerous, I need normal or control samples to compare with cancerous samples, can I combine control samples...

Asked on 01/20/2021 by Morteza Hadizadeh

1 answer

Understanding the fasta File Format

I'm a computer scientist teaching algorithms development in the Fall. One of the algorithms we teach is called Edit Distance, and our folklore is that it is used to compare...

Asked on 01/18/2021

2 answer

How to calculate various properties of a protein structure per atom using PDB2PQR and Rosetta tools (or any other tools)?

Currently, I am implementing a descriptor of protein structure and would like to calculate properties per atoms like charges, hydrophobicity, hydrogen bond donors / acceptors, hydrophobicity, aromaticity etc. I tried...

Asked on 01/17/2021 by Nurlybek Amangeldiuly

1 answer

how to calculate Pairwise Alignment Scores for blosum62

I have a bioinformatics exam coming up. I can understand the difference between the global algorithm and the local algorithm, but I have a problem with gap opening penalty and...

Asked on 01/16/2021 by Imren Bayil

1 answer

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