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Bioinformatics : Recent Questions and Answers (Page 34)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

Metagenomics: Identifying most common sequences

I am working on a project and used the following command:vsearch --derep_fulllength filtered_merged.fa -sizeout -relabel Uniq -output dereplicated_filtered_merged.faand got the following output:87373926 nt in 203453 seqs, min 310,...

Asked on 03/01/2021 by DumbledoreTheGrey

1 answer

Query on htseq count

This question has also been asked on Biostars I am trying to run htseq-count for carrying out rna-seq analysis for solanum tuberosum and i used the following...

Asked on 02/28/2021 by Lavanya C

0 answer

Why do I get cytosine to guanine/adenine transitions in bisulphite treated sequences?

I got my sequencing results (bisulphite treated and non treated sequences of same species Allium cepa) and now I have to do analysis in Cymate online tool. I prepared all...

Asked on 02/27/2021 by alimm94

0 answer

Can't locate object method "new" via package Bio::Tools::Primer3

Hi there I am still a novice at this. I'm trying to run this perl script called FullSSR https://sourceforge.net/projects/fullssr, by following the instructions in their 'README.TXT' ...

Asked on 02/25/2021 by ShawnC

1 answer

Multi Omics comparison RNA seq : Condition specific gene signature

The authors of an article are comparing different cell type and the organ type and they show the ontology enrichment of the RNA-seq:Cell-type-specific and organ-specific marker genes...

Asked on 02/25/2021

1 answer

Get nucleic acids' chain names from PDB API

There is a place in my pipeline where I call PDB's API with a ribosomal id to get all the subchains of that molecule programmatically I'd like to separate subchains...

Asked on 02/21/2021 by rtviii

0 answer

How to read E-value annotation on NCBI BLAST?

i am a bit confused how to read the current E value annotation on NCBI BLAST results. I looked into the matter but could not find an factual answer ....

Asked on 02/21/2021 by user12256545

0 answer

How are the scores of GeneSplicer, MaxEntScan and SpliceRegion interpreted from VEP annotations?

I am using VEP from Ensembl to annotate my VCF files with the extra plugins of GeneSplicer, MaxEntScan and ...

Asked on 02/20/2021 by Law

0 answer

Kraken2 or metaphlan2 report to phyloseq

I wish to convert a kraken2 or metaphlan2 report into the R package to analyze using Phyloseq. I have performed obtained an importation and Phyloseq analysis, but the result was...

Asked on 02/20/2021

0 answer

Genome assembly of SRR12196449 with SPAdes

I am trying to assemble the run SRR12196449 with SPAdes. The description of their project is:This project expected to standardize a method for amplification andsequencing of FIV genome in...

Asked on 02/19/2021

2 answer

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