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Bioinformatics : Recent Questions and Answers (Page 33)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

How do I find all supplementary mappings of a chimeric long (ONT) read mapped with NGMLR?

Unlike this guy How do I find split reads? I have done a lot of reading before asking this question. I'm aware that the FLAG will tell me if...

Asked on 03/10/2021 by Liam McIntyre

0 answer

Python - Finding a motif - input: a txt file with 10 sequences and 10 motifs

When I run my BruteForce function with only one input it works and the result is correct.def BruteForce(s, t): occurrences = [] ...

Asked on 03/07/2021 by Beeta

2 answer

How can I get properties from each one letter amino acid?

Im using python and wish to get properties from each one letter amino acid? I meanM(Met) => Density:1.340, MW:149.21... The RDkit methods atom.GetSymbol(), atom.GetDegree() permit this and I am...

Asked on 03/07/2021 by MH Lee

0 answer

error in random forest analysis

I am now struggling to do random forest analysis, I will be thankful if you could help with code for random forest analysis. I got samples from the root, soil,...

Asked on 03/07/2021

1 answer

Upgrading guppy basecaller on Oxford Nanopore Gridion via PPA

I am trying to update the guppy_basecaller (current version installed is 3.2.10+aabd4ec) on a ONT Gridion device. I am initially trying to update it via the nanopore PPA, as...

Asked on 03/05/2021 by BCArg

1 answer

Explanation for RNA-seq samples not clustering in PCA as expected

A colleague is analysing RNA-seq data - the study design is 2 treatments, 3 replicates, 3 tissues. In their PCA plot the samples clustered neatly by tissue. Except for two...

Asked on 03/05/2021 by user260878

1 answer

How to find possible viral subsequences in a Eukaryote genome?

I have an assembly of an algae and want to find if it has anything analogous to endogenous retroviruses' subsequences in its DNA. My guess is I should manually blastn...

Asked on 03/04/2021

2 answer

How to infer the number of chaperones involved in protein folding?

Let us suppose to have the complete CDS set of an organisms. Let us suppose to want to infer the number of chaperones involved in folding a certain subclass of...

Asked on 03/03/2021

0 answer

How to call this : some dots in volcano plot aligned a line after add a very small value to padj

when I plotting the volcano plot, I got some dots very top of the Y-axis because their padj nearly reached 0(I checked the data, I think they are reliable), so...

Asked on 03/01/2021 by Chu

0 answer

How to analyze the correlation of two different tissue gene expression under three conditions?

I have three conditions A, B, and C. For each state, I have gene expression data of two different tissues(liver and pancreas). I am studying the possible network/correlation/ between tissues...

Asked on 03/01/2021 by DowithR

0 answer

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