Bioinformatics Questions, Problems & Solutions : TransWikia.com ~ Page 32

What is a PacBio "movie file"?

I came across references to "movie files" from PacBio sequencing in this paper: https://www.jimmunol.org/content/204/12/3434 Specifically:Movie files used to generate results presented in this article have been submitted...

Asked on 03/17/2021

1 answer

Within and between sample count normalization

recently I came across a situation where RNASeq sample quality was expresses trough DESeq sizeFactor. So authors reported quality of their samples with respect to some publicly available datasets as...

Asked on 03/16/2021 by maxNo3

1 answer

How to perform KOG classification?

I have a list of DEGs and I want to perform KOG functional analysis, I try this method but WebMGA is not updated and...

Asked on 03/13/2021 by Mendel

0 answer

Is there a baby Hello World example using BIGstack?

Intel and the Broad Institute have developed BIGstack, described in this Broad-Intel Genomics stack whitepaper. It isn't clear how to run some simple example...

Asked on 03/13/2021

0 answer

Finding simple sequence from reads with significant overlap

I wrote a "script" to pull out reads from a huge fastq file in an iterative manner, by finding homology to the previous sequence. It should be relatively easy to...

Asked on 03/13/2021 by Ryan Ward

1 answer

Too slow issue of BioMart

I am using library(biomaRt) in R to annotate SNP rsid. It takes too much time for only 1000 SNPs....... I would like to know how I can run this code...

Asked on 03/12/2021 by user224050

1 answer

Question about public availability of human SNP dataset with country of origin

Is there a publicly available data set for humans SNPs, preferably together with the country of origin? If so, could someone please point me towards it. I've looked through other...

Asked on 03/12/2021 by user257566

0 answer

Obtaining Whole Genetic Sequence

As an end-user of my own data, specifically raw DNA sequence (WGS, whole genome sequencing). How or where do one obtain such DNA data so that a biology hobbyist can...

Asked on 03/11/2021

2 answer

Nvidia Parabrick fq2bam pipeline error - No such file or directory

I am trying to implement Nvidia-parabrick fq2bam pipeline to process my WES dataset. The reason for opting this pipeline is the huge number of samples (1004) which take many months...

Asked on 03/11/2021

1 answer

Does this shape has two clusters or one?

This cluster belongs to the direction of one side group from a long peptide during the simulation. I tried to make each cluster...

Asked on 03/10/2021

1 answer

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