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Bioinformatics : Recent Questions and Answers (Page 29)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

Number of edges in Graphical Analysis using Jupyter with Go Programming language

I'm trying to learn graphical analysis in a biological setting. The online course suggested using the Go Programming language, so I'm new to it and help to understand why the...

Asked on 04/07/2021

1 answer

Trying to create a .bam file without the need for a .sam file

I'm trying to use the code specified in this link to create a .bam file without the need for a .sam file. Here is the code I'm using:bwa...

Asked on 04/05/2021

2 answer

Comparing multiple treatments to multiple other treatments in edgeR for simple effects in a complex experimental design

I am working with a RNA-seq data set in maize that has a relatively complex design. There are two levels of treatment A (nitrogen fertilizer level in the field, high...

Asked on 04/04/2021

1 answer

NCBI blast for exact match of a short sequence

I'm trying to Blast for exact matches to the sequence: 'ATTGNNNNGCAAACCA' in the human transcriptome using NCBI Blast on its 'refseq_rna' database. However, when I do a basic query I...

Asked on 04/04/2021 by Mike Flynn

1 answer

Confirmation about PERMANOVA and Variance partitioning (permutest)

Could you please help me to understand what is the difference between Permutational multivariate analysis of variance (PERMANOVA,adonis) and Variance partitioning(permutest). I understood that permutest is used to...

Asked on 04/04/2021

1 answer

How do I obtain a vcf read from a pyvcf.Reader?

I wondered how would I be able to obtain variant based on chrom and location range. Optimally, this function would be like:import vcfvcf_reader = vcf.Reader(filename=some_file_name)vcf_read = vcf.variants_between(CHROM, START, END)Does...

Asked on 04/04/2021

1 answer

Basic question about finding mRNA sequence in transcriptome

I am a computer scientist just starting out in bioinformatics topics, and would appreciate any guidance that can be given here: I have an mRNA sequence- an...

Asked on 04/04/2021

2 answer

hisat2 --rna-strandness option and downstream htseq-count analysis

I've got some doubts on the hisat2 --rna-strandness option and its output for downstream analysis. Please see below. I understand that the --rna-strandness option produces an XS tag to indicate...

Asked on 04/04/2021 by guan

1 answer

gene-gene correlation from two different Tissues

I am stuck on how to do correlation for two independent data sets with common row and column names.A and B are datasets that contain as many rows as...

Asked on 04/03/2021

1 answer

Hybrid approach in metabolomics (NMR and MS)

Is it currently common in metabolomics to combine two quantification methods, namely NMR (nuclear magnetic resonance) and MS (mass spectrometry, either liquid or gas)? If so, what would be profits...

Asked on 04/02/2021 by Garrus990

0 answer

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