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Bioinformatics : Recent Questions and Answers (Page 26)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

How to make CIRCOS plot of VCF file?

I have 3 VCF files, I want to make CIRCOS plot just like the following: https://www.nature.com/articles/s41598-020-70527-8/figures/4 I try to follow the tutorial but could...

Asked on 05/01/2021

0 answer

lower mapping rates in salmon v0.13 compared to previous versions

Hi there :) Thanks for the tool! I recently updated to the new salmon (from 0.8... its been a couple years) and I noticed that my mapping percentages change dramatically...

Asked on 04/29/2021 by Courtney Stairs

1 answer

How to assign LD proxy

When we use local data using TwoSampleMR, how can we assign LD proxies where the outcome data is not available? https://mrcieu.github.io/TwoSampleMR/articles/outcome.html outcome_dat <- read_outcome_data( ...

Asked on 04/28/2021

0 answer

How to know if ID correspond to nucleotide sequence or protein with a script

I have a text file that contains a list of IDs (314 sequences):AVP78031.1AVP78042.1ATO98108.1ATO98120.1ATO98132.1...My goal is to make a script (maybe using Python or Perl) to check in the list...

Asked on 04/27/2021 by Mauri1313

1 answer

How to demultiplex a mix of single-indexed and dual-indexed samples

The problemIf I have a sample sheet that contains both single-indexed and dual-indexed samples, I can split it up into two sample sheets and then run bcl2fastq on each one....

Asked on 04/27/2021 by AmadeusDrZaius

1 answer

Assign cell types to groups of cells based on their gene expression profiles

I have large filtered, normalized dataset of scRNA-seq data of C.Elegans species. Rows are genes (10 000), columns are cells (66 000). Let's say that I got 40 different groups...

Asked on 04/26/2021 by Nikita Vlasenko

2 answer

How is BLAST's nr database created?

Is there a paper or web page describing the procedure for creating the nr database used by NCBI's BLAST implementation? I presume it's some type of clustering, but I'm curious...

Asked on 04/25/2021 by juniper-

2 answer

summarising read group information from a .bam file

I have merged together 2 different .bam files in order to simulate sample contamination. So the reads can come from one of two samples, as shown by the read group...

Asked on 04/25/2021 by user438383

0 answer

Will using smaller kmers help get larger contigs? If not, then what?

I've been using MEGAHIT to assemble metagenomes, with particular focus on specific genomic areas. Sometimes all I get is gene fragments or pathway fragments (eg. if I know that genes...

Asked on 04/25/2021

1 answer

Using dssp after chain extraction

I have a list of PDB IDs with realtive chains that are to be extracted, and the run on dssp.For the single chain extraction I tried several methods, such...

Asked on 04/24/2021 by saiden

0 answer

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