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Bioinformatics : Recent Questions and Answers (Page 22)

Find answers to your questions about Bioinformatics or help others by answering their Bioinformatics questions.

MM/PB(GB)SA calculations for DESMOND trajectories

I did molecular dynamic simulations with DESMOND. Now, I want to obtain the interaction free energy using MM/PB(GB)SA. The free tools I found to perform such calculations are ...

Asked on 06/02/2021 by user9085

0 answer

Compute the significance of the overlap between 2 or more gene sets

I was able to compute the significance of the overlap between 2 gene sets using the cdf function of scipy hypergeometric distribution. I wish to be able...

Asked on 05/31/2021

1 answer

Alternative to enrichR for enrichment analysis?

I have been using EdgeR from the Bioconductor package to perform differential expression analysis on bulk RNASeq data sets. This analysis gives me an output dataframe that...

Asked on 05/30/2021 by Nova

2 answer

Publicly available genome sequence database for viruses?

As a small introductory project, I want to compare genome sequences of different strains of influenza virus. What are the publicly available databases of influenza virus gene/genome sequences?...

Asked on 05/30/2021 by AlwaysTrying44

3 answer

Create GFF3 feature exporter. The information is below:

Problem StatementThe GFF3 format is a commonly-used one in bioinformatics for representing sequenceannotation. You can find the specification here: http://www.sequenceontology.org/gff3.shtml The genome and annotation for...

Asked on 05/29/2021 by Kendal B.

1 answer

Annotating gene names or gene IDs to a dataframe containing SNPs?

I have a large data-frame (excel file) of SNPs with genotyping data. I need to filter the data to get SNP information of a specific gene alone. The list is...

Asked on 05/29/2021

1 answer

creating a tab delimited file

I am working on a project using a fasta file. I am writing my command in nano within command-line and executing using python, also within my command-line. I would like...

Asked on 05/29/2021 by Edwardo

2 answer

Does rRNA depletion protocol give higher number of mapped reads in Intronic regions?

Recently, I have downloaded a publicly available dataset, which are 350 tumor samples. I see the following information from the published paper. ...

Asked on 05/28/2021

2 answer

Add tags to the read pairs in a bam file

I have a bam file from paired-end sequencing, in which each R1 has the RX tag assigned (via UMItools group) denoting a Unique Molecular Identifier (UMI). I want to call...

Asked on 05/27/2021 by SPPearce

1 answer

Power law distribution alpha values

I did a Pearson co-expression analysis for generating networks for my tissue-specific (chondrocytes) RNA-seq data. I used R package poweRlaw to check for the power-law distribution. We got the following...

Asked on 05/25/2021

1 answer

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