Physics Asked on February 3, 2021
When a light microscope is used in the reflected mode there is a semi-transparent mirror angled at 45 degrees that reflects a portion of the light onto the sample. The sample in turn reflects the light coming off the beam and it goes straight through the 45 degrees angled semi-transparent mirror.
How is it that in this process we don’t lose half the photons from the beam of the light source?
In effect, you loose half the light intensity, but normally, that is still enough.
Answered by trula on February 3, 2021
You lose half the light twice, each time the light passes through the half-silvered mirror: Once heading toward the sample, and once again heading back. This leaves you with only one-quarter of the original light intensity reaching the eyepiece. This is why reflection-mode microscopes have to use very powerful bulbs to get enough illumination.
Answered by niels nielsen on February 3, 2021
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