How to create a map to PNG file with specific box coordinates by Mapnik?

I recently manually built a tile server with this instruction ->
https://switch2osm.org/serving-tiles/manually-building-a-tile-server-20-04-lts/.
I downloaded location data for Russia from geofabrik.de and then loaded to database by osm2pgsql.
Now all working nicely – Apache, mod_tile, renderd, Mapnik and PostgreSQL with PostGIS.

Now I want to create a program on Python for generating a PNG file with specific region (box). This region will be defined by coordinates (left and right corners of box).
I write this code:

import mapnik

mapnik_xml = "openstreetmap-carto/mapnik.xml"
map_output = "region_map_mapnikXml.png"

# Create a map object
m = mapnik.Map(600,300)
mapnik.load_map(m, mapnik_xml)
bbox = mapnik.Box2d(45.23, 41.34, 46.23, 43.23)
m.zoom_to_box(bbox)
mapnik.render_to_file(m, map_output)
print(f"Rendered image to {map_output}")

But after executing this code created only blank file with background.
I don’t figure out why?
Can you help me please.

Additionaly, if I run this code:

import mapnik

mapnik_xml = "openstreetmap-carto/mapnik.xml"
map_output = "world_map_mapnikXml.png"

# Create a map object
m = mapnik.Map(600,300)
mapnik.load_map(m, mapnik_xml)
m.zoom_all()
mapnik.render_to_file(m, map_output)
print(f"Rendered image to {map_output}")

in results, generated PNG file – world map with shape of mainlands and countries.

Why is this happening?

from pyproj import Proj, transform

inProj = Proj('epsg:4326')
outProj = Proj('epsg:3857')

x1,y1 = -105.150271116, 39.7278572773
x2,y2 = transform(inProj,outProj,x1,y1)
print(x2,y2)