a scap of , a spot of and a trace of

No, you can't.

A spot of bother is an expression, meaning a little trouble, although the expression is often used ironically to mean a lot of trouble.

A scrap of..... (or a few scraps of) is generally, but not necessarily, used for food, indicating a very small amount.

A scrap - without the preposition - can also refer to a fight.

A trace of.... is most often used in reporting tiny amounts of a substance, especially those found on clothes or in medicines, compounds, food recipes and so on.

These distinctions are typical rather than required but, regarding your query, to write a scrap/trace of bother would not be idiomatic.