TransWikia.com

TMB vs ECL for ELISA: which detection method is more sensitive?

Biology Asked by Miko on October 22, 2021

I failed to find a comparison, does anyone know which ELISA detection method is more sensitive of these two: ECL, TMB?

Many thanks!

One Answer

In general, ECL is more sensitive than TMB for ELISA detection. The minimum detection limit of TMB is 60pg/mL and 20pg/mL for ultra TMB [1], whereas the detection limit of ECL can be as low as about 1.7pg/mL [2].

Resources:

  1. https://info.gbiosciences.com/blog/elisa-substrates-a-selection-guide

  2. https://www.thermofisher.cn/cn/zh/home/life-science/antibodies/immunoassays/elisa-kits/elisa-enzyme-substrates.html

Answered by Leading Biology on October 22, 2021

Add your own answers!

Ask a Question

Get help from others!

© 2024 TransWikia.com. All rights reserved. Sites we Love: PCI Database, UKBizDB, Menu Kuliner, Sharing RPP