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Is there a chemical reaction that blocks two cysteines by reacting with a third molecule?

Biology Asked by Antoine Roland on December 31, 2020

The idea is to block the two cysteines so they can’t react in the future. We need the reaction to remove the -SH groups of the two cysteines, or modify them.

Also important, the reaction should not make cross-link between the two cysteines.

bi-Cysteine -SH group passivation reaction. Ideas for an adapted chemical ?

To explain briefly my motivation: I am a PhD student and I do optical tweezer experiments. We can hold and manipulate two micrometer beads in two optical traps. We have a process to tether one protein using two DNA linkers to the two beads. I need to block two of the three cysteines of my trimer to properly couple the protein to both beads.

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