High pressure cryonics

Question

In this link, it is suggested to use high-pressure cryonics to freeze living cells, tissues or small organism as opposed to various and potentially toxic anti-freeze agent. The core idea is that over a certain pressure, ice is anamorphic and will not form ice crystals. Thus, organic manner will not be destroyed by said crystals.

Detailed information about the proposal can be found at this link:

https://www.benbest.com/cryonics/pressure.html

I have considering self-financing the experiment. Could anyone chip in with some advice? Do you see any red flags, design tips, etc.?

Dr. H. Lecter · Answer

One premise of the full proposal is that the freezing process should avoid the formation water crystals which prevent structural damage of membranes and proteins and keep the integrity of the life organism being frozen under high-pressure cryonics.

I have not seen a discussion on the thawing process which I always thought was a problem for example when thawing cells for in vitro cell culture.

For the design I would try small with for example mammalian cells like HeLa. Maybe full organisms like worms or flies for "tissues" and "full organisms".

High pressure cryonics

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