Biology Asked on June 2, 2021
If I’m right, there are two types of pluripotent stem cells: naïve and prime. The naïve form is more undifferentiated.
It would seem to me that it would be easier to make genetically engineered animals if some kind of mutation were made in the cells and the cells that could successfully produce the mutation were iPS-transformed and injected into the blastocyst. But, I don’t know of any transgenic animal that has been created in that way, with the exception of the mouse.
Recently, common method seems to be to administer Crisper Cas9 directly into the blastocyst.
My question:
- For which animals, can we make iPS-derived reproductive chimeras?
- Preferably, the chimeras can produce offspring derived from genetically modified iPS.
- However, the question is about animals other than mice.
It would be great if you could also tell me about the following;
- What are the strains of animals that have been genetically modified in this way?
- If it is difficult to create genetically modified animals in this way, what are the barriers?
- However, the questions are also about animals other than mice.
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