Bioinformatics Asked on December 23, 2021
samtools depth
to find the per base-pair read coverage over a number of isoform contigs from my Trinity assembly.In my final step, I want to assess the per-residue read coverage of the consensus amino acid sequence to demonstrate an idea of confidence in that sequence.
Does anyone have any tips on how to go about doing this and whether there are existing tools for such things?
Answer from @cephbirk, converted from comment:
What I ended up doing was to choose the read coverage for the lowest covered nucleotide in each codon as each residue's read coverage.
Answered by gringer on December 23, 2021
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