Bioinformatics Asked by Neeleshwar Pandey on December 1, 2020
from Bio import SeqIO
from Bio import Align
ref_seq_1 = SeqIO.read('C:/Users/King_Arthur/Desktop/ref_seq/segment 1/ref_seq_8.fasta','fasta')
seq1 = SeqIO.read('C:/Users/King_Arthur/Desktop/file/segment 1/Myfile_1 (1).fasta','fasta')
aligner = Align.PairwiseAligner()
aligner.mode = 'global'
aligner.match_score = 1
aligner.mismatch_score = -2
aligner.gap_score = -2
alignments = aligner.score(ref_seq_1.seq , seq1.seq)
print(alignments)
for alignment in sorted(alignments):
print(alignment)
So this is my code and as you can see in the last section i am trying to iterate over my alignment but I am getting this error
TypeError: 'float' object is not iterable
I have tried various things like using str()
but it gives some strange values and I also tried to read the source code by using the inspect
module but I can’t figure out the problem.
Any help would be really appreciated.
My final objective is to find out how many matches, mismatches and gaps are present in the final alignment using biopython.
if there is any other better way to do it in python please feel free to suggest.
aligner.score(ref_seq_1.seq , seq1.seq)
doesn't produce an alignment. It just gives you the score of the alignment as float value.
Instead you need to run:
alignments = aligner.align(ref_seq_1.seq , seq1.seq)
Now alignments
is an Bio.Align.PairwiseAlignments
you can iterate over alignments
or get the score with alignments.score
which gives you the same as aligner.score(ref_seq_1.seq , seq1.seq)`
If you need more information, you could check the documentation.
Correct answer by Mr_Z on December 1, 2020
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