Bioinformatics Asked by user977828 on January 10, 2021
I ran HiSat2, MarkDuplicate, removed reads with the lower quality score than 40 and finally only kept properly paired reads.
After the BAM filtering steps, I used the Scallop results with TransDecoder. Next, I used bamCoverage to create RNA-Seq profile. I noticed that not always the first isoform is the best one compared to the RNA-Seq profile as could be seen below a few examples:
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By any chance, is there a way to keep only the best isoform which is in red box?
Thank you in advance,
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