Biology Asked by Maximilian Press on December 30, 2020
I have recently been getting more interested in amateur botany, and have some background in genetics and genomics (PhD+). I have used standard light and confocal microscopes but don’t have much expertise in microscopy.
I often find myself wondering what the ploidy is of some plant that I’m looking at, as I know that this is a major life history feature and genetic variant in wild plants.
I was curious whether a cheap little scope like a foldscope would be an adequate microscopy instrument for looking at the cytogenetics of a (probably rather crudely prepared) plant sample, assuming that I can come up with a suitable home protocol. (I don’t currently have access to lab space.)
I have found this question, but it doesn’t really explain much about the required parameters for cytogenetic microscopy.
So:
1) What microscopy parameters need to be satisfied to do cytogenetics?
2) Does a foldscope meet them?
3) If not a foldscope, what other cheapo scope would work?
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